Proteome integrity is safeguarded by the Proteostasis Network (PN), an intricately regulated network of conserved processes that have evolved to regulate native protein structure, localization, function and turnover in order to maintain proteome balance. Proteostasis imbalances, deficiencies and collapse are increasingly implicated in a range of human disorders. Pro2 represents a novel integrated analytical platform enabling the intuitive exploration of the characteristics and extent of PN deregulation in human diseases. Pro2 unites tools for the assessment of gene expression changes of PN functional processes with interactive visualization applications, such as heat maps, polar plots, and interactome-guided 3D topographic landscapes (‘topologies’). The provided ontology covers all major PN processes ordered by more top-level down to detailed functional groups, such as the human chaperome and its changes across 22 solid cancers represented in The Cancer Genome Atlas (TCGA), as described in this study. Furthermore, Pro2 accepts upload and analysis of custom gene expression data for the analysis of PN alterations in user-specific sample data.